Journal Published Online: 01 March 1991
Volume 36, Issue 2

Genetic Markers in Human Bone: I. Deoxyribonucleic Acid (DNA) Analysis

CODEN: JFSCAS

Abstract

Deoxyribonucleic acid (DNA) was isolated from a number of spongy and compact human bone tissue specimens, and the yield was estimated on a “per milligram of starting tissue” basis. DNA was, in addition, isolated from a number of corresponding blood and bone tissue specimens. Spectrophotofluorometry and ethidium bromide visualization on minigels were used to estimate the quantity and degree of degradation of DNA. The DNA from several blood-bone pairs is shown to give concordant restriction fragment length polymorphism (RFLP) typing results by two different typing protocols with five different singlelocus probes. DNA from several additional blood-bone pairs is shown to give concordant results for human leucocyte antigen (HLA)-DQα phenotypes following polymerase chain reaction (PCR) amplification and hybridization to specific allele-specific oligonucleotide (ASO) probes, and for the variable numbers of tandem repeats (VNTR) length polymorphisms 3′ to the human apolipoprotein B (APOB) gene following PCR amplification with specific primers and analysis of the products by electrophoresis and ethidium bromide visualization.

Author Information

Lee, HC
Yale University School of Medicine, New Haven, CT
Pagliaro, EM
Yale University School of Medicine, New Haven, CT
Berka, KM
Office of Coroner/Medical Examiner, Los Angeles, CA
Folk, NL
Office of Coroner/Medical Examiner, Los Angeles, CA
Anderson, DT
Office of Coroner/Medical Examiner, Los Angeles, CA
Ruano, G
Yale University School of Medicine, New Haven, CT
Keith, TP
Collaborative Research, Inc., Bedford, MA
Phipps, P
Collaborative Research, Inc., Bedford, MA
Herrin, GL
DNA Laboratory, Forensic Science Division, Georgia Bureau of Investigation, Decatur, GA
Garner, DD
DNA Laboratory, Forensic Science Division, Georgia Bureau of Investigation, Decatur, GA
Gaensslen, RE
Office of Coroner/Medical Examiner, Los Angeles, CA
Pages: 11
Price: $25.00
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Stock #: JFS13034J
ISSN: 0022-1198
DOI: 10.1520/JFS13034J