Journal Published Online: 01 May 1990
Volume 35, Issue 3

Hae III—A Suitable Restriction Endonuclease for Restriction Fragment Length Polymorphism Analysis of Biological Evidence Samples

CODEN: JFSCAS

Abstract

Hae III has been selected by our laboratories as the restriction endonuclease of choice for restriction fragment length polymorphism analysis of forensic science samples. The enzyme is compatible with the D2S44 probe system and generates relatively small DNA fragments for that marker system. Similarly, Hae III is compatible with several other independent polymorphic loci, including D1S7, D4S139, D16S85, D17S74, D17S79, D14S13, and D20S15. Hae III is functional under a variety of adverse conditions for DNA digestion and is not affected by the methylation pattern in mammals. Finally, Hae III is a relatively inexpensive restriction endonuclease.

Author Information

Budowle, B
Forensic Science Research and Training Center, FBI Academy, Quantico, VA
Waye, JS
Royal Canadian Mounted Police, Central Forensic Laboratory, Ottawa, ON, Canada
Shutler, GG
Royal Canadian Mounted Police, Central Forensic Laboratory, Ottawa, ON, Canada
Samuel Baechtel, F
Forensic Science Research and Training Center, FBI Academy, Quantico, VA
Pages: 7
Price: $25.00
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Stock #: JFS12858J
ISSN: 0022-1198
DOI: 10.1520/JFS12858J