Journal Published Online: 01 September 1989
Volume 34, Issue 5

Sex Determination of Bloodstains by the Use of a Ribonucleic Acid (RNA) Probe

CODEN: JFSCAS

Abstract

An asymmetric ribonucleic acid (RNA) probe, which represents a Y-chromosome specific nucleotide sequence, was applied to the sex origin of bloodstains by using a nonisotopic Southern hybridization method. The RNA probe was in vitro transcribed from the recombinant of the Y-chromosome specific deoxyribonucleic acid (DNA) sequence and transcription-vector Bluescript SK M13+. The use of this RNA probe can lower the detection limit of the nonisotopic DNA analyzing method to ⅛ of that reached by our previously reported method in which a DNA probe was used.

Author Information

Kobayashi, R
Juntendo University School of Medicine, Tokyo, Japan
Matsuzawa, S
Juntendo University School of Medicine, Tokyo, Japan
Pages: 4
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Stock #: JFS12743J
ISSN: 0022-1198
DOI: 10.1520/JFS12743J