Journal Published Online: 01 September 1989
Volume 34, Issue 5

Evaluation of Deoxyribonucleic Acid (DNA) Isolated from Human Bloodstains Exposed to Ultraviolet Light, Heat, Humidity, and Soil Contamination

CODEN: JFSCAS

Abstract

This study was designed to analyze the effects of common environmental insults on the ability to obtain deoxyribonucleic acid (DNA) restriction fragment-length polymorphisms (RFLP) patterns from laboratory prepared specimens. The environmental conditions studied include the exposure of dried bloodstains to varying amounts of relative humidity (0, 33, 67, and 98%), heat (37°C), and ultraviolet light for periods of up to five days. In addition, the effect of drying over a four-day period in whole blood collected with and without ethylenediaminetetraacetate (EDTA) was examined.

The results of the study showed that, under the conditions studied, the integrity of DNA is not altered such that false RFLP patterns are obtained. The only effect observed was that the overall RFLP pattern becomes weaker, but individual RFLP fragments are neither created nor destroyed.

Author Information

McNally, L
Lifecodes Corporation, Valhalla, NY
Shaler, RC
Lifecodes Corporation, Valhalla, NY
Baird, M
Lifecodes Corporation, Valhalla, NY
Balazs, I
Lifecodes Corporation, Valhalla, NY
Forest, PD
John Jay College of Criminal Justice, New York, NY
Kobilinsky, L
John Jay College of Criminal Justice, New York, NY
Pages: 11
Price: $25.00
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Stock #: JFS12741J
ISSN: 0022-1198
DOI: 10.1520/JFS12741J