Journal Published Online: 01 July 1989
Volume 34, Issue 4

Assay of Free and Total Tocainide by High Performance Liquid Chromatography (HPLC) with Ultraviolet (UV) Detection

CODEN: JFSCAS

Abstract

A rapid high performance liquid chromatographic (HPLC) method for the determination of tocainide, using N-(2,6-dimethylphenyl)-2-amino-butanimide as an internal standard, was developed. A methylene chloride extraction involving salting out at pH 9.0 was employed. An 85:15 mixture of 0.025M monobasic potassium phosphate at pH 3.0 and acetonitrile was used as the mobile phase. The separation and quantitative analysis of tocainide was performed on a mixed phase column with a 1.0-mL/min flow rate and detection at 210 nm. Separation of tocainide from some of its metabolites required the use of heptane sulfonic acid as an ionpairing reagent. For the free-drug assay, the specimen was centrifuged through an Amicon Centrifree filter before being processed.

Author Information

Harris, SC
Veterans Administration Medical Center, Battle Creek, MI
Guerra, C
University of Texas Health Science Center, San Antonio, TZ
Wallace, JE
University of Texas Health Science Center, San Antonio, TZ
Pages: 6
Price: $25.00
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Stock #: JFS12720J
ISSN: 0022-1198
DOI: 10.1520/JFS12720J