Journal Published Online: 01 May 1988
Volume 33, Issue 3

A Hybrid Ampholyte Focusing Technique for Esterase D Subtyping of Evidentiary Material

CODEN: JFSCAS

Abstract

An ultrathin-layer polyacrylamide gel isoelectric focusing technique that uses a composite of ampholytes from three commercial sources is described for subtyping esterase D. All common allelic products of esterase D were separated clearly. The technique described in this paper provides a higher conclusive call rate on known blood specimens (95.8%) and questioned bloodstains (69.7%) compared with continuous zone electrophoresis in agarose gels (89.9 and 37.6%, respectively).

Author Information

Budowle, B
Forensic Science Research and Training Section, FBI Academy, Quantico, VA
Gambel, AM
Forensic Science Research and Training Section, FBI Academy, Quantico, VA
Pages: 6
Price: $25.00
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Stock #: JFS12482J
ISSN: 0022-1198
DOI: 10.1520/JFS12482J