Journal Published Online: 01 May 1988
Volume 33, Issue 3

Identification of Male Bloodstains by Dot Hybridization of Human Y Chromosome-Specific Deoxyribonucleic Acid (DNA) Probe

CODEN: JFSCAS

Abstract

The sex determination of bloodstains was performed using a human Y chromosome-specific (DNA) fragment of 1.9-kb length as a hybridization probe. The DNA samples were taken from 1- and 4-week-old bloodstains of males and females, respectively. Strong signals with male DNA were observed by Y-probe, while faint signals with female DNA were detected. In addition, clear signals were observed in the extract samples from male bloodstains (16-week-old) on paper. Dot hybridization of the Y-probe would be widely applicable to studies on sex determination of medicolegal materials such as blood, bloodstains, teeth, and cadaverous parts.

Author Information

Fukushima, H
Shinshu University School of Medicine, Nagano, Japan
Hasekura, H
Shinshu University School of Medicine, Nagano, Japan
Nagai, K
Tokyo Medical College, Tokyo, Japan
Pages: 7
Price: $25.00
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Stock #: JFS12471J
ISSN: 0022-1198
DOI: 10.1520/JFS12471J