Journal Published Online: 01 July 1987
Volume 32, Issue 4

Lewis Typing of Human Bloodstains by Enzyme-Linked Immunosorbent Assay (ELISA) Using Monoclonal Anti-Le and Anti-Le

CODEN: JFSCAS

Abstract

The Lewis blood grouping of human dried bloodstains could be determined by an enzyme-linked immunosorbent assay (ELISA) using monoclonal anti-Lea and anti-Leb antibodies with an avidin-biotin complex (ABC). The bloodstains aged 1 year were used as samples, and approximately 1 mg of the stains was enough to type each Lewis antigen reliably by this method. The Lewis substances of 106 individual stains were correctly typed regardless of their ABO blood group system.

Author Information

Takatori, T
Hokkaido University School of Medicine, Sapporo, Japan
Tustsubuchi, Y
Hokkaido University School of Medicine, Sapporo, Japan
Terazawa, K
Hokkaido University School of Medicine, Sapporo, Japan
Pages: 6
Price: $25.00
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Stock #: JFS12401J
ISSN: 0022-1198
DOI: 10.1520/JFS12401J