Journal Published Online: 01 April 1986
Volume 31, Issue 2

Screening for Barbiturates in Vitreous Humor by the EMIT®-st Serum Enzyme Immunoassay

CODEN: JFSCAS

Abstract

In 16 medical examiner's cases, which were found to be barbiturate-positive by thin-layer chromatographic screening of the liver, blood barbiturate concentrations were determined by gas chromatography. The corresponding vitreous humor samples were screened by the enzyme multiplied immunoassay technique, the EMIT®-st serum barbiturate assay. By using the recommended dilution for detecting serum barbiturates, it was possible to detect barbiturates in vitreous humor at a toxic concentration. By using one fourth the amount of diluent, the barbiturates could be detected also at a therapeutic concentration. The EMIT-st assay proved to be useful in the screening for barbiturates in vitreous humor, a material that is readily available in forensic toxicology.

Author Information

Ojanperä, I
University of Helsinki, Helsinki, Finland
Vuori, E
University of Helsinki, Helsinki, Finland
Nieminen, R
University of Helsinki, Helsinki, Finland
Penttilä, A
University of Helsinki, Helsinki, Finland
Pages: 3
Price: $25.00
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Stock #: JFS12303J
ISSN: 0022-1198
DOI: 10.1520/JFS12303J