Journal Published Online: 01 April 1986
Volume 31, Issue 2

A Microplate Method for Reverse ABO Typing of Bloodstains

CODEN: JFSCAS

Abstract

A sensitive and reliable hemagglutination assay, using V-bottom microplates, is described for the detection of the ABO blood group alloantibodies in bloodstained material. When used in conjunction with an absorption-elution procedure, the microplate assay resulted in a 300% increase in the number of conclusive grouping results when compared to the Lattes crust test. The use of the microplate reverse grouping assay permits 24 specimens to be assayed conveniently on a single plate and eliminates the tedious and time-consuming microscopic examination required for the Lattes crust test.

Author Information

Mudd, JL
FBI Academy FSRTC, Quantico, VA
Pages: 8
Price: $25.00
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Stock #: JFS12271J
ISSN: 0022-1198
DOI: 10.1520/JFS12271J