Journal Published Online: 01 April 1986
Volume 31, Issue 2

Analysis of Restriction Fragment Length Polymorphisms in Deoxyribonucleic Acid (DNA) Recovered from Dried Bloodstains

CODEN: JFSCAS

Abstract

Deoxyribonucleic acid (DNA) was recovered from dried bloodstains aged up to three years and shown to be of high molecular weight. DNA was digested with restriction endonucleases and fractionated by agarose gel electrophoresis. Following transfer to a filter, DNA was hybridized with two different radioactively labeled recombinant probes which recognize highly polymorphic regions in human DNA. The autoradiographic pattern observed was not altered by sample age, and the size of the alleles was consistent with those observed in the general population. Therefore, DNA of high molecular weight prepared from dried blood samples can be used for identification.

Author Information

Kanter, E
Lifecodes Corp., Elmsford, NY
Baird, M
Lifecodes Corp., Elmsford, NY
Shaler, R
Office of the Chief Medical Examiner, New York, NY
Balazs, I
Lifecodes Corp., Elmsford, NY
Pages: 6
Price: $25.00
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Stock #: JFS12269J
ISSN: 0022-1198
DOI: 10.1520/JFS12269J