Journal Published Online: 01 July 1982
Volume 27, Issue 3

Determination of -methamphetamine in Urine after Administration of - or -methamphetamine to Rats by Radioimmunoassay Using Optically Sensitive Antiserum

CODEN: JFSCAS

Abstract

A radioimmunoassay was developed for the determination of d-methamphetamine in urine. Antiserum to d-methamphetamine was prepared in rabbits by immunization with d-N-4-aminobutylmethamphetamine conjugated with bovine serum albumin, d-1-[3H]-Methamphetamine was used as a labeled compound for radioimmunoassay. The specificity of the antibody against d-methamphetamine was determined by cross-reaction studies with optical isomers of methamphetamine and its analogs. The antibody was specific for d-methamphetamine and exhibited no significant cross-reaction with thel-isomers. This stereoselective assay was applied to determination of d-methamphetamine excreted in urine after oral administration of d- or dl-methamphetamine to rats.

Author Information

Niwaguchi, T
National Research Institute of Police Science, Tokyo, Japan
Kanda, Y
National Research Institute of Police Science, Tokyo, Japan
Kishi, T
National Research Institute of Police Science, Tokyo, Japan
Inoue, T
National Research Institute of Police Science, Tokyo, Japan
Pages: 6
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Stock #: JFS12171J
ISSN: 0022-1198
DOI: 10.1520/JFS12171J