Journal Published Online: 01 October 1986
Volume 31, Issue 4

Subtyping Phosphoglucomutase-1 in Semen Stains and Bloodstains: A Report on the Method

CODEN: JFSCAS

Abstract

A method is described for obtaining nondistorted, reproducible phosphoglucomutase-1 subtyping patterns from semen stains and bloodstains. Isoelectric focusing of phosphoglucomutase-1 was accomplished in 80 min in a 0.2-mm-thick polyacrylamide gel with an interelectrode wick distance of 8.0 cm. The gel contained 1.2% (w/v) N-(2-hydroxyethyl) piperazine-N-3-propanesulfonic acid (EPPS) and pH 5 to 7 ampholytes (4% w/v). When maintained at room temperature, laboratory-prepared bloodstains and semen stains could be typed for phosphoglucomutase-1 up to four months and three weeks, respectively. An evaluation of phosphoglucomutase-1 typing by isoelectric focusing and the Group I system was performed on casework samples submitted to the FBI Laboratory. In addition to the increased discriminating probability of phosphoglucomutase-1 when subtyped, isoelectric focusing yielded an increase in positive calls on questioned bloodstains (65.6 versus 36.2%) and dried seminal stains (16.4 versus 13.1%) compared with the Group I system.

Author Information

Budowle, B
Forensic Science Research and Training Center, Laboratory Division, FBI Academy, Quantico, VA
Murch, RS
Serology Unit, Scientific Analysis Section, Laboratory Division, Federal Bureau of Investigation, Washington, DC
Davidson, LC
Forensic Science Research and Training Center, Laboratory Division, FBI Academy, Quantico, VA
Gambel, AM
Serology Unit, Scientific Analysis Section, Laboratory Division, Federal Bureau of Investigation, Washington, DC
Kearney, JJ
Forensic Science Research and Training Center, Laboratory Division, FBI Academy, Quantico, VA
Pages: 8
Price: $25.00
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Stock #: JFS11911J
ISSN: 0022-1198
DOI: 10.1520/JFS11911J