Journal Published Online: 01 October 1984
Volume 29, Issue 4

Increasing the Sensitivity of Detection of the Electrophoretic Marker Group Specific Component in Agarose Gels by Double-Staining with Coomassie Brilliant Blue R250 and Silver

CODEN: JFSCAS

Abstract

A method is described that increases the sensitivity of detection of group-specific component (Gc) in bloodstains following agarose gel electrophoresis. Gels are stained first with Coomassie Brilliant Blue R250 (CB) and subsequently stained with silver. Double-staining with CB first enhances the sensitivity of the silver stain from 10- to 80-fold over staining with CB alone. The method is reproducible and can easily be incorporated into the laboratory as a routine procedure.

Author Information

Budowle, B
Forensic Science Research Group, Laboratory Division, FBI Academy, Quantico, VA
Pages: 4
Price: $25.00
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Stock #: JFS11788J
ISSN: 0022-1198
DOI: 10.1520/JFS11788J