Journal Published Online: 01 April 1984
Volume 29, Issue 2

Liquid Chromatographic Analysis of Promethazine and Its Major Metabolites in Human Postmortem Material

CODEN: JFSCAS

Abstract

A method is described for the determination of promethazine and some of its major metabolites in postmortem specimens by enzymic digestion followed by high-pressure liquid chromatography of the extracts using a cyano-bonded (μ-Bondapak®-CN) silica column packing and a mobile phase consisting of methanol, water, and n-octylamine (adjusted to pH 8). The system will separate and quantitate promethazine sulphoxide, desmonomethyl promethazine, didesmethyl promethazine, and promethazine. Pericyazine (2-cyano-10-[3-(4-hydroxypiperidino)propyl] phenothiazine) was used as internal standard. The parent drug and metabolites were extracted from a enzyme digested tissue homogenates with ethyl acetate using a simple, single micro-extraction procedure. The method was applied to four cases of fatal poisoning involving promethazine ingestion.

Author Information

Allender, WJ
Forensic Toxicology Section, Department of Health New South Wales, Lidcombe, N.S.W., Australia
Archer, AW
Research Section, Department of Health New South Wales, Lidcombe, Australia
Pages: 12
Price: $25.00
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Stock #: JFS11699J
ISSN: 0022-1198
DOI: 10.1520/JFS11699J