Journal Published Online: 01 November 1987
Volume 32, Issue 6

The Use of Separator Isoelectric Focusing in Micro-Ultrathin Polyacrylamide Gels in the Characterization of Some Polymorphic Proteins of Forensic Science Significance

CODEN: JFSCAS

Abstract

The identification of phenotypes of erythyrocyte acid phosphatase (EAP), esterase D (EsD), group specific component (Gc), and alpha-1-antitrypsin (PI) by separator isoelectric focusing in micro-ultrathin polyacrylamide gels (interelectrode distance: 45 mm) is described. The protein patterns obtained are compared favorably with the patterns seen by isoelectric focusing in conventional polyacrylamide gel dimensions (interelectrode distance: 110 to 120 mm). The technique described allows greater stability of pH gradients and is a fast and economic method.

Author Information

Alonso, A
Biology Section, National Institute of Toxicology, Madrid, Spain
Gascó, P
Biology Section, National Institute of Toxicology, Madrid, Spain
Pages: 7
Price: $25.00
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Stock #: JFS11215J
ISSN: 0022-1198
DOI: 10.1520/JFS11215J