Journal Published Online: 01 July 1986
Volume 31, Issue 3

The Use of Ultrathin-Layer Agarose Gels for Phenotyping Erythrocyte Acid Phosphatase by Isoelectric Focusing

CODEN: JFSCAS

Abstract

A method is described for the use of ultrathin-layer agarose gels in phenotyping erythrocyte acid phosphatase (EAP) by isoelectric focusing (IEF). The results obtained using ultrathin-layer agarose gels are shown to be equally reliable and reproducible in comparison to established ultrathin-layer polyacrylamide gels. IEF of EAP on 0.168-mm agarose gels took place in 90 min using the LKB Multiphor system. The technique described allows for both time and cost efficient phenotyping of EAP.

Author Information

Frank, WE
Illinois Department of State Police, Bureau of Forensic Sciences, Training and Applications Laboratory, Joliet, IL
Stolorow, MD
Illinois Department of State Police, Bureau of Forensic Sciences, Training and Applications Laboratory, Joliet, IL
Pages: 6
Price: $25.00
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Stock #: JFS11117J
ISSN: 0022-1198
DOI: 10.1520/JFS11117J