Journal Published Online: 01 January 1980
Volume 25, Issue 1

The Simultaneous Identification of Seminal Acid Phosphatase and Phosphoglucomutase by Starch Gel Electrophoresis

CODEN: JFSCAS

Abstract

Although elevated acid phosphatase (AP) activity in vaginal fluid is a consistent indicator for semen, differentiation between vaginal AP and seminal AP provides a more meaningful result. Detection of seminal AP in mixtures of vaginal AP, feces, and blood is accomplished by starch gel electrophoresis, employing the substrate thymolphthalein monophosphate as a selective visualization agent. Genetic phosphoglucomutase isoenzymes are simultaneously separated by this method and allow differentiation in some semen/vaginal fluid mixtures.

Author Information

Linde, HG
Vermont State Police Laboratory, Montpelier
Molnar, KE
BCI East Central Laboratory, Cambridge, Ohio
Pages: 5
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Stock #: JFS10945J
ISSN: 0022-1198
DOI: 10.1520/JFS10945J