Journal Published Online: 01 January 1980
Volume 25, Issue 1

The Preparation of Lipid-Free Extracts of Drugs from Blood for Gas Chromatographic Analysis

CODEN: JFSCAS

Abstract

A method is presented to separate co-extracted biological materials, in particular lipids, from drugs in chloroform extracts of blood. The chloroform is evaporated and the residue is dissolved in a small volume of petroleum ether and partitioned with eight times this volume of 5% aqueous hydrochloric acid. The acidic, basic, and neutral drugs are extracted into the aqueous phase, leaving unwanted materials in the petroleum ether. The recoveries of drugs representative of a variety of types are presented. Reduction of the levels of lipids by factors on the order of 1000-fold is reported.

Author Information

Tye, R
Hamilton County Coroner's Office, Cincinnati, Ohio
Freitag, J
Hamilton County Coroner's Office, Cincinnati, Ohio
Pages: 4
Price: $25.00
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Details
Stock #: JFS10942J
ISSN: 0022-1198
DOI: 10.1520/JFS10942J