ASTM WK70106

    New Guide for Standard Guide to Assess Microbial Decontamination of Indoor Air using an Aerobiology Chamber

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    Developed by Subcommittee: E35.15 | Committee E35 | Contact Staff Manager


    1. Scope

    This guide is to assess technologies for microbial decontamination of indoor air using a sealed, room-sized chamber (~24 m3) as recommended by the U.S. Environmental Protection Agency. The test microbe is aerosolized inside the chamber where a fan uniformly mixes the aerosols and keeps them airborne. Samples of the air are collected and assayed, firstly to determine the rates of natural physical and biological decay of the test microbe, and then to assess the air decontaminating activity of the technology under test as log10 or percentage reductions in viability per cubic meter. The air temperature and relative humidity (RH) in the chamber are measured and recorded during each test.


    Aerosols; aerobiology; Acinetobacter baumannii; Klebsiella pneumoniae; Pseudomonas aeruginosa; Staphylococcus aureus; decontamination; nebulization; indoor air; soil load; surrogate.


    Indoor air can spread pathogens, and many technologies are either already on the market or under development for microbial decontamination of indoor air. However, suitable test methods remain unavailable to assess such technologies. This guide addresses the gap.

    The title and scope are in draft form and are under development within this ASTM Committee.

    Citing ASTM Standards
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    Work Item Status

    Date Initiated:

    Technical Contact:
    Syed Sattar


    E35 (21-03)

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