1.1 This test method describes a standardized method of processing cellulose wipes of environmental surfaces to detect Bacillus anthracis spores sampled from nonporous surfaces and provide a semi-quantitative estimate of the amount of B. anthracis contamination after a release has been detected. 1.2 This test method should be performed in a BSL3 laboratory by those trained for BSL3 microbiological techniques. 1.3 This test method is specific to B. anthracis but could be adapted for use with other organisms. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
Keywordsanthrax; Bacillus anthracis; spores; surface sampling; wipe sampling; wipe processing; environmental sampling
Currently, no test method exists to process environmental samples from surfaces using wipes. This procedure describes a standardized, optimized test method of processing cellulose wipes of environmental surfaces to detect B. anthracis spores sampled from nonporous surfaces and provide a semi-quantitative estimate of the amount of B. anthracis contamination after a release been detected. This test method will be used by laboratories responding to a release of B. anthracis spores, indoors or outdoors, to characterize the initial spread and determine if decontamination has been effective. This test method can also be adapted for other organisms, such as drug-resistant healthcare-associated organisms (MDROs, such as MRSA, Clostridium difficile spores, Acinetobacter baumannii,and Enterococcus faecalis). Future guidelines are planned to address use of this test method for MDROs of interest in investigating healthcare-associated outbreaks.Back to Top