ASTM WK63310

    New Guide for Visualization and Identification of Nanoparticles in Cells Using Darkfield Microscopy with Hyperspectral Imaging Analysis

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    Developed by Subcommittee: E56.08 | Committee E56 | Contact Staff Manager


    1. Scope

    1.1The guide contains general information and has been prepared in order to familiarize laboratory scientists with the background and content necessary to image and identify engineered nanoparticles in mammalian cells using darkfield microscopy/hyperspectral imaging (DFM/HSI). 1.2The guide covers the recommended criteria and considerations for performing DFM/HSI analyses on mammalian cells containing engineered nanoparticles. 1.3DFM/HSI is a relatively new analytical tool and is increasingly being used for diverse applications in nanomedicine, nanotoxicology and environmental nanotechnology. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    biodistribution; biological; carbon nanotubes; metals; metal oxides; semiconductor quantum dots; semiquantitation; spectrophotometry; uptake;


    Darkfield microscopy/hyperspectral imaging (DFM/HSI) is a relatively new analytical technique for identifying and characterizing the distribution of engineered nanoparticles in biological tissues and other matrices with refractive indices near that of water. DFM/HSI requires minimal sample preparation and is capable of much higher sample throughputs than established techniques, such as SEM-EDX or TEM-EDX, for identifying nanoparticles in complex matrices, and has the benefit of providing both a visual high-contrast and spectroscopic identification Continued rapid advances in basic biomedical research and in pharmaceutical research and development are dependent upon correctly identifying and characterizing the uptake of nanoparticles into cells as well as being able to identify nanoscale domains within complex dosage forms such as emulsions and suspensions. Similarly, it is important to characterize and identify nanoparticles in cells when trying to understand the fate and distribution of nanoparticles in the environment. The developed guide will enable both academic and industrial researchers to design experiments that can be used to correctly identify and characterize nanoparticles in complex matrices with a solid understanding of the potential benefits and limitations of the technology. The guide will also provide academic and industry researchers with a roadmap for interpretation of the accumulated hyperspectral data. The main users of the guide will be academic and government labs engaged in biomedical research, Pharm R&D corporations, and government agencies that either regulate drug and cosmetic products containing nanodomains (FDA) or agencies that have jurisdiction over nanomaterial environmental health and safety (EPA).

    The title and scope are in draft form and are under development within this ASTM Committee.

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    Work Item Status

    Date Initiated:

    Technical Contact:
    Bryant Nelson

    Draft Under Development