ASTM WK61243

    New Test Method for Immunological Assay to Quantify Extractable Guayule Natural Rubber (GNR) Proteins

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    Developed by Subcommittee: D11.40 | Committee D11 | Contact Staff Manager



    WK61243

    1. Scope

    This test method covers an immunological method to determine the amount of extractable protein in natural rubber and its products using rabbit antisera specific for Guayule Natural Rubber latex (GNR) proteins. This immunoassay procedure quantitatively measures the level of GNR proteins in solution using an inhibition format. The samples may include but are restricted to, gloves or other GNR product extracts which have been collected in order to measure the protein levels. Although this method detects antigenic proteins, it should not be considered as a measure of allergenic proteins. Correlation of protein/antigen levels with the level of allergenic proteins has not been fully established.


    Keywords

    ELISA; GNR Guayule Natural Rubber; proteins

    The title and scope are in draft form and are under development within this ASTM Committee.

    Citing ASTM Standards
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    Work Item Status

    Date Initiated:
    11-27-2017

    Technical Contact:
    David Kostyal

    Item:
    004

    Ballot:
    D11 (18-05)

    Status:
    Ballot Item Approved