ASTM WK54615

    New Guide for Standard Practice for Performing Electron Cryo-Microscopy of Liposomes.

    (What is a Work Item?)

    Developed by Subcommittee: E56.02 | Committee E56 | Contact Staff Manager


    1. Scope

    This practice covers procedures for preparing and recording images of a suspension or suspensions of liposomes for the purpose of evaluating their shape and size distribution for quality assessment. The sample is to be vitrified in a liquid ethane slush onto specially prepared holey carbon electron microscope (EM) grids, and imaged in a cryo-holder placed in a cryo-capable electron microscope.

    Liposomes make up ~26% of nano-related drug submissions. Some guidance is needed in the area of characterization.


    electron microscopy nanotechnology size shape liposome

    The title and scope are in draft form and are under development within this ASTM Committee.

    Citing ASTM Standards
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    Work Item Status

    Date Initiated:

    Technical Contact:
    Angel Paredes


    E56 (17-03)

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