ASTM WK54615

    New Guide for Standard Practice for Performing Electron Cryo-Microscopy of Liposomes.

    (What is a Work Item?)

    Developed by Subcommittee: E56.02 | Committee E56 | Contact Staff Manager


    1. Scope

    This practice covers procedures for preparing and recording images of a suspension or suspensions of liposomes for the purpose of evaluating their shape and size distribution for quality assessment. The sample is to be vitrified in a liquid ethane slush onto specially prepared holey carbon electron microscope (EM) grids, and imaged in a cryo-holder placed in a cryo-capable electron microscope.


    electron microscopy nanotechnology size shape liposome


    Liposomes make up ~26% of nano-related drug submissions. Some guidance is needed in the area of characterization.

    Citing ASTM Standards
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    Work Item Status

    Date Initiated:

    Technical Contact:
    Hany Demian