ASTM WK45351

    New Test Method for Quantitative Evaluation of Antibacterial Performance of Porous Antibacterial Treated Articles

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    Developed by Subcommittee: E35.15 | Committee E35 | Contact Staff Manager


    1. Scope

    1. Scope 1.1 To determine the bactericidal or bacteriostatic properties of porous articles treated with an active biocidal agent, samples of porous treated materials, such as textiles or paper, are inoculated with a defined suspension of microorganisms and then incubated. The changes in numbers of the bacterial populations on the treated article are compared with untreated articles either over designated time or they are compared to the initial bacterial population at zero time for the treated article to measure antibacterial properties. 1.2 This method is used for measuring the quantitative antibacterial activity of porous materials that have been treated with a biocide to inhibit the growth of bacteria on the treated materials. This method may also be used to measure the ability of the treated material to inhibit the growth of a microorganism. It can measure both bactericidal and bacteriostatic activity. 1.3 This method shall be performed by individuals experienced and adept in microbiological procedures and in facilities suitable for the handling of the microorganisms under test. 1.4 This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

    This test method will be used to determine the antibacterial properties of articles, particularly textiles or non-wovens treated with specific antimicrobial agents. Other such similar standards do exist in the industry, but these methods either offer variables that affect intralaboratory reproducibility or limit the utility of using these methods to demonstrate a variety of end-use performance claims.


    percent bacterial reduction, quantitative antibacterial assay, bactericidal, bacteriostatic, porous, porous substrates, paper, textile, treated articles, antimicrobial treated surfaces.

    The title and scope are in draft form and are under development within this ASTM Committee.

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    Work Item Status

    Date Initiated:

    Technical Contact:
    Robert Monticello


    E35.15 (17-02)

    Negative Votes Need Resolution