ASTM WK28779

    New Test Method for Single Tube Extraction Method (STEM) for Sporicidal Fumigants and Liquid Disinfectants

    (What is a Work Item?)

    Developed by Subcommittee: E54.03 | Committee E54 | Contact Staff Manager


    1. Scope

    1.1 This test method determines the efficacy of liquid disinfectant and gaseous fumigant agent on microbial cells and spores dried on the surface of a variety of hard porous and non-porous carriers. 1.2 This test method can be used to evaluate antimicrobial formulations (or decontaminant, disinfectant, and so forth), purported to have sporicidal and/or bactericidal activity. This test method allows: 1.2.1 Use of variable size coupons (5 mm to 10 mm size circles and squares of 2 4 mm thickness). 1.2.2 Quantifying the sporicidal efficacy of different antimicrobial formulations and gases; 1.2.2 Identifying the effect, if any, of the material combination on sporicidal efficacy; and 1.2.3 Comparing the relative resistance of microbial cells and spores to different levels of sporicidal treatment 1.3 The values stated in SI units are to be regarded as the standard. 1.4 Strict adherence to the protocol is necessary for the validity of the test results. 1.5 Follow all the safety guidance of the institution in which the testing is being conducted. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

    A laboratory test method to determine the efficacy of sporicidal fumigants and liquid disinfectants is needed that has more flexibility and usability than the method currently available: E 2414.


    STEM, quantitative test method, sporicidal efficacy, spore recovery from complex surfaces

    The title and scope are in draft form and are under development within this ASTM Committee.

    Citing ASTM Standards
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    Work Item Status

    Date Initiated:

    Technical Contact:
    Robert Knowlton

    Draft Under Development