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1.1 This practice is designed to evaluate antimicrobial agents for the prevention of microbially influenced deterioration of liquid fuels (as defined by Specification D 396, D 910, D 975, D 1655, D 2069, D 2880, D 3699, D 4814, D 6227, and D 6751), system deterioration, or both.
1.2 Knowledge of microbiological techniques is required for these procedures.
1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practice (GLP) is required and to follow them where appropriate (40 CFR, 160), or as revised.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
D396 Specification for Fuel Oils
D910 Specification for Leaded Aviation Gasolines
D975 Specification for Diesel Fuel Oils
D1655 Specification for Aviation Turbine Fuels
D2069 Specification for Marine Fuels
D2880 Specification for Gas Turbine Fuel Oils
D3699 Specification for Kerosine
D4814 Specification for Automotive Spark-Ignition Engine Fuel
D5465 Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
D6227 Specification for Unleaded Aviation Gasoline Containing a Non-hydrocarbon Component
D6293 Test Method for Oxygenates and Paraffin, Olefin, Naphthene, Aromatic(O-PONA) Hydrocarbon Types in Low-Olefin Spark Ignition Engine Fuels by Gas Chromatography
D6469 Guide for Microbial Contamination in Fuels and Fuel Systems
D6729 Test Method for Determination of Individual Components in Spark Ignition Engine Fuels by 100 Metre Capillary High Resolution Gas Chromatography
D6733 Test Method for Determination of Individual Components in Spark Ignition Engine Fuels by 50-Metre Capillary High Resolution Gas Chromatography
D6751 Specification for Biodiesel Fuel Blend Stock (B100) for Middle Distillate Fuels
D6974 Practice for Enumeration of Viable Bacteria and Fungi in Liquid FuelsFiltration and Culture Procedures
D7463 Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
D7464 Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
D7467 Specification for Diesel Fuel Oil, Biodiesel Blend (B6 to B20)
D7687 Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
D7978 Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water--Thixotropic Gel Culture Method
E1326 Guide for Evaluating Non-culture Microbiological Tests
Federal Standards40 CFR Part 152 Pesticide Registration and Classification Procedures 40 CFR Part 79 Fuels and Fuel Additives Registration Regulations Available from U.S. Government Printing Office Superintendent of Documents, 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401.
UNSPSC Code 51102700(Antiseptics); 47131803(Household disinfectants)
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ASTM E1259-05, Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C, ASTM International, West Conshohocken, PA, 2005, www.astm.orgBack to Top