ASTM D8070-16

    Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay

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    1. Scope

    1.1 This test method describes a procedure that can be used in the field or in a laboratory to detect antigens indicative of microbial contamination in liquid fuels, including those blended with synthesized hydrocarbons or biofuels, with kinematic viscosities (at 40 °C) of 24 mm2s–1 (for example, Specifications D396, D975, and D1655) and in fuel-associated water.

    1.1.1 This test method has been validated by an ILS for a range of middle distillate fuels meeting Specification D1655, EN590, Specification D975, and ISO 8217:2012.

    1.2 This test method semi-quantitatively assesses the concentration of specific antigens generated by aerobic microorganisms during active growth in fuels.

    1.2.1 A proprietary formulation of antibodies and antibody mixtures is used to detect three types of microbial antigen contamination: antigens generally found in aerobic bacteria, antigens generally present in common fungi (yeast and molds), and an antigen that is characteristic of Hormoconis resinae (the fungus most commonly associated with fuel biodeterioration).

    1.2.2 Although the antibodies and antibody mixtures are characteristic of diverse types of bacteria and fungi, it is unlikely that they are universal. Recognizing that for every microbe that has been isolated and characterized, it is likely that there are a billion that have not. Consequently, as is the case with all microbiological test methods, this test method does not purport to detect 100 % of the microbes present in a fuel or fuel-associated water sample.

    1.3 For each of the three sets of antigen detected (H. resinae, common fungi, and aerobic bacteria), the test detects whether the antigen concentration present is within set ranges representing negligible, moderate, or heavy microbial contamination.

    1.3.1 For fuel specimens, the antigen concentration ranges detected are <150 µg/L (negligible), 150 µg/L to 750 µg/L (moderate), and >750 µg/L (heavy).

    1.3.2 For specimens of water associated with fuel, the antigen concentration ranges detected are <33 µg/mL (negligible), 33 µg/mL to 166 µg/mL (moderate), and >166 µg/mL (heavy).

    1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For a specific hazard statement, see Section 8.

    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D396 Specification for Fuel Oils

    D975 Specification for Diesel Fuel

    D1129 Terminology Relating to Water

    D1655 Specification for Aviation Turbine Fuels

    D4175 Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants

    D4176 Test Method for Free Water and Particulate Contamination in Distillate Fuels (Visual Inspection Procedures)

    D6469 Guide for Microbial Contamination in Fuels and Fuel Systems

    D6974 Practice for Enumeration of Viable Bacteria and Fungi in Liquid FuelsFiltration and Culture Procedures

    D7464 Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing

    D7687 Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration

    D7847 Guide for Interlaboratory Studies for Microbiological Test Methods

    D7978 Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated WaterThixotropic Gel Culture Method

    E1326 Guide for Evaluating Non-culture Microbiological Tests

    E2756 Terminology Relating to Antimicrobial and Antiviral Agents

    Other Standards

    BS EN590 Standard for Diesel Fuel Available from British Standards Institution (BSI), 389 Chiswick High Rd., London W4 4AL, U.K.,

    IATA Guidance Material on Microbiological Contamination in Aircraft Fuel Tanks, Fifth Edition, 2015 Available from International Aviation Transport Association (IATA) at

    NATO Logistics Handbook

    ICS Code

    ICS Number Code 75.160.20 (Liquid fuels)

    UNSPSC Code

    UNSPSC Code 15101500(Petroleum and distillates)

    Referencing This Standard
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    DOI: 10.1520/D8070-16

    Citation Format

    ASTM D8070-16, Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay, ASTM International, West Conshohocken, PA, 2016,

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