ASTM D7463-08

    Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel Associated Water


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    1. Scope

    1.1 This test method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with:

    1.1.1 Microorganisms found in conventional liquid fuels with kinematic viscosities (at 40°C) of 8 mm2 · s1 as described in Table X6.1,

    1.1.2 Microorganisms found in fuel-associated bottom water, and

    1.1.3 Extracellular (non-cellular) ATP present in the sample matrix.

    1.2 The ATP is measured using a patented bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the sample. The light is produced and measured quantitatively using dedicated ATP test pens and a dedicated luminometer and reported in (instrument specific) Relative Light Units.

    1.3 This test method is equally suitable for use in the laboratory or field.

    1.4 Although bioluminescence is a reliable and proven method for qualifying and quantifying ATP, this method does not differentiate between ATP from different sources, for example, from different types of microorganism such as bacteria or fungi.

    1.5 For water or capture solution samples, the concentration range of ATP detectable by this test method is 1 × 1011 M to 3 × 108 M which is equivalent to 1 × 1014 moles/mL to 3 × 1011 moles/mL for water samples or capture solution. Assuming testing on fuel phase is performed on a 500 mL volume of fuel the equivalent concentrations is fuel would be: 6 × 1011 M to 2 × 1014 M.

    1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D396 Specification for Fuel Oils

    D975 Specification for Diesel Fuel Oils

    D1655 Specification for Aviation Turbine Fuels

    D2880 Specification for Gas Turbine Fuel Oils

    D4012 Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water

    D4175 Terminology Relating to Petroleum Products, Liquid Fuels, and Lubricants

    D6300 Practice for Determination of Precision and Bias Data for Use in Test Methods for Petroleum Products and Lubricants

    D7464 Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing

    D7467 Specification for Diesel Fuel Oil, Biodiesel Blend (B6 to B20)


    ICS Code

    ICS Number Code 07.100.99 (Other standards related to microbiology); 75.160.20 (Liquid fuels)

    UNSPSC Code

    UNSPSC Code 15101500(Petroleum and distillates)


    Referencing This Standard
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    DOI: 10.1520/D7463-08

    Citation Format

    ASTM D7463-08, Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel Associated Water, ASTM International, West Conshohocken, PA, 2008, www.astm.org

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