ASTM E685 - 93(2013)

    Standard Practice for Testing Fixed-Wavelength Photometric Detectors Used in Liquid Chromatography

    Active Standard ASTM E685 | Developed by Subcommittee: E13.19

    Book of Standards Volume: 03.06


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    Significance and Use

    4.1 Although it is possible to observe and measure each of the several characteristics of a detector under different and unique conditions, it is the intent of this practice that a complete set of detector specifications should be obtained under the same operating conditions. It should also be noted that to completely specify a detector's capability, its performance should be measured at several sets of conditions within the useful range of the detector. The terms and tests described in this practice are sufficiently general that they may be used regardless of the ultimate operating parameters.

    4.2 Linearity and response time of the recorder or other readout device used should be such that they do not distort or otherwise interfere with the performance of the detector. This requires adjusting the gain, damping, and calibration in accordance with the manufacturer's directions. If additional electronic filters or amplifiers are used between the detector and the final readout device, their characteristics should also first be established.

    1. Scope

    1.1 This practice is intended to serve as a guide for the testing of the performance of a photometric detector (PD) used as the detection component of a liquid-chromatographic (LC) system operating at one or more fixed wavelengths in the range 210 to 800 nm. Measurements are made at 254 nm, if possible, and are optional at other wavelengths.

    1.2 This practice is intended to describe the performance of the detector both independently of the chromatographic system (static conditions) and with flowing solvent (dynamic conditions).

    1.3 For general liquid chromatographic procedures, consult Refs (1-9).2

    1.4 For general information concerning the principles, construction, operation, and evaluation of liquid-chromatography detectors, see Refs (10 and 11) in addition to the sections devoted to detectors in Refs (1-7).

    1.5 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    E275 Practice for Describing and Measuring Performance of Ultraviolet and Visible Spectrophotometers

    E682 Practice for Liquid Chromatography Terms and Relationships


    ICS Code

    ICS Number Code 17.180.30 (Optical measuring instruments); 71.040.50 (Physicochemical methods of analysis)

    UNSPSC Code

    UNSPSC Code 41115311(Photometers)


    DOI: 10.1520/E0685-93R13

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