ASTM E394 - 09

    Standard Test Method for Iron in Trace Quantities Using the 1,10-Phenanthroline Method

    Active Standard ASTM E394 | Developed by Subcommittee: E15.01

    Book of Standards Volume: 15.05


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    ASTM E394

    Significance and Use

    This test method is suitable for determining trace concentrations of iron in a wide variety of products, provided that appropriate sample preparation has rendered the iron and sample matrix soluble in water or other suitable solvent (see 10.1 and Note 6).

    This test method assumes that the amount of color developed is proportional to the amount of iron in the test solution. The calibration curve is linear over the specified range. Possible interferences are described in Section 5.

    1. Scope

    1.1 This test method covers the determination of iron in the range from 1 to 100 μg.

    1.2 This test method is intended to be general for the final steps in the determination of iron and does not include procedures for sample preparation.

    1.3 This test method is applicable to samples whose solutions have a pH less than 2. It is assumed that the pH is adjusted to within this range in the sample preparation.

    1.4 Review the current material safety data sheets (MSDS) for detailed information concerning toxicity, first-aid procedures, handling, and safety precautions.

    1.5 The values given in SI units are the standard. Values in parentheses are for information only.

    1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1193 Specification for Reagent Water

    E60 Practice for Analysis of Metals, Ores, and Related Materials by Spectrophotometry

    E180 Practice for Determining the Precision of ASTM Methods for Analysis and Testing of Industrial and Specialty Chemicals

    E200 Practice for Preparation, Standardization, and Storage of Standard and Reagent Solutions for Chemical Analysis

    E275 Practice for Describing and Measuring Performance of Ultraviolet and Visible Spectrophotometers


    ICS Code

    ICS Number Code 71.040.40 (Chemical analysis)

    UNSPSC Code

    UNSPSC Code 41116105(Chemistry reagents or solutions)


    DOI: 10.1520/E0394-09

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