ASTM E2657 - 11

    Standard Test Method for Determination of Endotoxin Concentrations in Water-Miscible Metalworking Fluids

    Active Standard ASTM E2657 | Developed by Subcommittee: E34.50

    Book of Standards Volume: 11.03


    Buy Standard (PDF)
    4 pages
    $37.00
    Buy Standard (Print)
    4 pages
    $37.00
    Buy Standard + Redline (PDF)
    8 pages
    $44.40

    Historical (view previous versions of standard)


    ASTM E2657

    Significance and Use

    The determination of endotoxin concentrations in metalworking fluids is a parameter that can be used in decision-making for prudent fluid management practices (fluid draining, cleaning, recharging or biocide dosages).

    This standard provides a test method for analysts who perform quantitative endotoxin analyses of water-miscible metalworking fluids.

    1. Scope

    1.1 This test method covers quantitative methods for the sampling and determination of bacterial endotoxin concentrations in water miscible metalworking fluids (MWF).

    1.2 Users of this test method need to be familiar with the handling of MWF.

    1.3 This method gives an estimate of the endotoxin concentration in the sampled MWF.

    1.4 This method replaces Test Method E2250.

    1.5 This test method seeks to minimize inter-laboratory variation of endotoxin data but does not ensure uniformity of results.

    1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D4840 Guide for Sample Chain-of-Custody Procedures

    E1497 Practice for Selection and Safe Use of Water-Miscible and Straight Oil Metal Removal Fluids

    E1542 Terminology Relating to Occupational Health and Safety

    Government Standard

    29CFR1910.1450 Occupational Exposure to Hazardous Chemicals in Laboratories


    ICS Code

    ICS Number Code 07.100.99 (Other standards related to microbiology); 75.100 (Lubricants, industrial oils and related products)

    UNSPSC Code

    UNSPSC Code


    DOI: 10.1520/E2657-11

    ASTM International is a member of CrossRef.

    ASTM E2657

    Citing ASTM Standards
    Back to Top