ASTM E2657 - 16

    Standard Practice for Determination of Endotoxin Concentrations in Water-Miscible Metalworking Fluids

    Active Standard ASTM E2657 | Developed by Subcommittee: E34.50

    Book of Standards Volume: 11.03

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    Significance and Use

    5.1 The determination of endotoxin concentrations in MWF is a parameter that can be used in decision-making for prudent fluid management practices (fluid draining, cleaning, recharging or biocide dosages).

    5.2 This standard provides a practice for analysts who perform quantitative endotoxin analyses of water-miscible MWF.

    1. Scope

    1.1 This practice covers quantitative methods for the sampling and determination of bacterial endotoxin concentrations in water miscible metalworking fluids (MWF).

    1.2 Users of this practice need to be familiar with the handling of MWF.

    1.3 This practice gives an estimate of the endotoxin concentration in the sampled MWF.

    1.4 This practice replaces Method E2250.

    1.5 This practice seeks to minimize inter-laboratory variation of endotoxin data but does not ensure uniformity of results.

    1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D4840 Guide for Sample Chain-of-Custody Procedures

    E1488 Guide for Statistical Procedures to Use in Developing and Applying Test Methods

    E1497 Practice for Selection and Safe Use of Water-Miscible and Straight Oil Metal Removal Fluids

    E1542 Terminology Relating to Occupational Health and Safety

    E2250 Method for Determination of Endotoxin Concentration in Water Miscible Metal Working Fluids

    Government Standard

    29 CFR 1910.1450 Occupational Exposure to Hazardous Chemicals in Laboratories

    ICS Code

    ICS Number Code 07.100.99 (Other standards related to microbiology); 75.100 (Lubricants, industrial oils and related products)

    UNSPSC Code

    UNSPSC Code

    Referencing This Standard
    Link Here
    Link to Active (This link will always route to the current Active version of the standard.)

    DOI: 10.1520/E2657-16

    ASTM International is a member of CrossRef.

    Citation Format

    ASTM E2657-16, Standard Practice for Determination of Endotoxin Concentrations in Water-Miscible Metalworking Fluids, ASTM International, West Conshohocken, PA, 2016,

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