ASTM E2657

The determination of endotoxin concentrations in metalworking fluids is a parameter that can be used in decision-making for prudent fluid management practices (fluid draining, cleaning, recharging or biocide dosages).

This test method provides a test method for analysts who perform quantitative endotoxin analyses of water-miscible metalworking fluids.

1. Scope

1.1 This test method covers quantitative methods for the sampling and determination of bacterial endotoxin concentrations in water miscible metalworking fluids (MWF).

1.2 Users of this test method need to be familiar with the handling of MWF.

1.3 This test method gives an estimate of the endotoxin concentration in the sampled MWF.

1.4 This test method replaces E 2250.

1.5 This test method seeks to minimize inter-laboratory variation of endotoxin data but does not ensure uniformity of results.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

2. Referenced Documents

Government Standard
































Other Documents
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
NIOSH Manual of Analytical Methods (NMAM), 4th ed., Eller and Cassinelli, Eds., 1994
ASTM Standards
D2881 Classification for Metal Working Fluids and Related Materials
D4840 Guide for Sample Chain-of-Custody Procedures
E1497 Practice for Selection and Safe Use of Water-Miscible and Straight Oil Metal Removal Fluids
E1542 Terminology Relating to Occupational Health and Safety

Index Terms

bioaerosols; endotoxin; endotoxin assay; endotoxin units (EU); Gram-negative bacteria; Limulus amebocyte lysate (LAL); metalworking fluids; pyrogen; ICS Number Code 07.100.99 (Other standards related to microbiology); 75.100 (Lubricants, industrial oils and related products)

Citing ASTM Standards

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