ASTM E2458 - 10

    Standard Practices for Bulk Sample Collection and Swab Sample Collection of Visible Powders Suspected of Being Biological Agents from Nonporous Surfaces

    Active Standard ASTM E2458 | Developed by Subcommittee: E54.01

    Book of Standards Volume: 15.08


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    Significance and Use

    These practices should be used only to collect visible samples that are suspected biothreat agents and have been field screened as defined by the FBI-DHS-HHS/CDC Coordinated Document for explosive hazard, radiological hazard, and other acute chemical hazards.

    1. Scope

    1.1 These practices address collection of visible powders that are suspected biothreat agents from solid nonporous surfaces using a bulk collection method, using a dry swab and laminated card, followed by a swab sampling method using a sterile moistened swab. Bulk powder samples are collected and packaged in a manner that permits the maximum amount of the sample to be safely transported to a reference laboratory within the Centers for Disease Control and Prevention (CDC) national Laboratory Response Network (LRN) for confirmatory identification and safe storage. If the source of the powder is a letter or small package, that item is also packaged in a manner that permits it to be safely transported to an LRN reference laboratory. A sterile moistened swab may be used to collect residual powder and may be used to conduct on-site biological assessments for the purpose of testing for biothreat agents.

    1.2 These practices are performed in coordination with the Federal Bureau of Investigation (FBI) as part of a risk assessment including hazard assessment and threat evaluation as recommended and clarified in Guide . The decision to implement these practices and collect a public safety sample will be made by members of the response community of the jurisdiction assuming responsibility through coordination with the FBI and the receiving LRN reference laboratory.

    1.3 Sample Collection Method A covers the bulk collection and packaging of suspicious visible powders that are suspected biothreat agents from solid nonporous surfaces. All samples suspected to be biothreat agents on nonporous surfaces should be collected according to Sample Collection Method A and sent to a LRN reference laboratory for confirmatory testing.

    1.4 Sample Collection Method B covers swab sampling of residual suspicious powders that are suspected biothreat agents from solid nonporous surfaces. Swab samples can be used for on-site biological assessment; however results from on-site biological assessments are not definitive; confirmatory testing by the LRN reference laboratory is necessary to make public health decisions.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    E2770 Guide for Operational Guidelines for Initial Response to a Suspected Biothreat Agent

    F2412 Test Methods for Foot Protection

    F2413 Specification for Performance Requirements for Protective (Safety) Toe Cap Footwear

    Federal Government Regulations

    OSHA-29CFR1910.1200 Hazard Communication


    ICS Code

    ICS Number Code 13.300 (Protection against dangerous goods); 71.040.40 (Chemical analysis); 87.040 (Paints and varnishes)

    UNSPSC Code

    UNSPSC Code


    DOI: 10.1520/E2458-10

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