ASTM E2315 - 03(2008)

    Standard Guide for Assessment of Antimicrobial Activity Using a Time-Kill Procedure

    Active Standard ASTM E2315 | Developed by Subcommittee: E35.15

    Book of Standards Volume: 11.05


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    ASTM E2315

    Significance and Use

    This procedure may be used to assess the in vitro reduction of a microbial population of test organisms after exposure to a test material.

    1. Scope

    1.1 This guide covers examples of a basic method to measure the changes of a population of aerobic microorganisms within a specified sampling time when tested against antimicrobial test materials in vitro. Several options for organism selection and growth, inoculum preparation, sampling times and temperatures are provided. When the basic technique is performed as a specific test method it is critical when evaluating the results to ensure that such variables have been standardized. Antimicrobial activity of specific materials, as measured by this technique, may vary significantly depending on variables selected. It is important to understand the limitations of in vitro tests, especially comparisons of results from tests performed under different circumstances. As an example, test results of microorganisms requiring growth supplements, or special incubation conditions, may not be directly comparable to more robust organisms under the conditions of a single procedure.

    1.2 Knowledge of microbiological techniques is required for this test.

    1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.4 This standard may involve hazardous materials, operations and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory requirements prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1193 Specification for Reagent Water

    E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents


    ICS Code

    ICS Number Code 07.100.99 (Other standards related to microbiology)

    UNSPSC Code

    UNSPSC Code 60104000(Biotechnology and bio chemistry and genetics and microbiology and related materials)


    DOI: 10.1520/E2315-03R08

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