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Significance and Use
5.1 The procedure in this test method is used to evaluate the activity of a test reagent (antimicrobial agent/active ingredient) or formulation in the reduction or complete kill of the bacterial population in fabric and wash water following a single wash. The water to fabric ratio in common top loading machines is dynamic and varies by region. The proper water to fabric ratio and temperature for the worse-case scenario should be determined prior to testing. This test method may need to modified if the worse-case scenario is determined to be in top loading high efficiancy washing machine
1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in traditional top-loading automatic clothes washing operations. This test method is designed predominantly to provide testing with representative vegetative bacteria but can also be designed to accommodate the testing of fungi and viruses.
Note 1: This test method does not evaluate sanitizing/disinfectant laundry detergent/additives for use in front-loading or top-loading, low water high efficiency automatic clothes washing operations.
1.2 This test method is intended to compliment Test Method and is to be used in the cases where this test method is determined to be the worse case scenario for product usage.
1.3 Knowledge of microbiological techniques is required for these procedures.
1.4 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow them where appropriate (see section 40 CFR, 160 or as revised.)
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
Note 2: In this test method, metric units are used for all applications, except for distance in which case inches are used.
1.6 Appropriate modifications to the test method may be required when the testing organisms are not specified herein.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
D1193 Specification for Reagent Water
E177 Practice for Use of the Terms Precision and Bias in ASTM Test Methods
E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents
Other DocumentsDIS/TSS 13 Laundry Additives--Disinfection and Sanitization, U.S. Environmental Protection Agency, Office of Pesticide Programs, April 1980 Available from United States Environmental Protection Agency (EPA), Ariel Rios Bldg., 1200 Pennsylvania Ave., NW, Washington, DC 20460, http://www.epa.gov.
ICS Number Code 71.100.35 (Chemicals for industrial and domestic desinfection purposes)
UNSPSC Code 47131803(Household disinfectants)
|Link to Active (This link will always route to the current Active version of the standard.)|
ASTM E2274-16, Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants, ASTM International, West Conshohocken, PA, 2016, www.astm.orgBack to Top