This method can be used to evaluate effectiveness of incorporated/bound antimicrobials in hydrophobic materials such as plastics, epoxy resins, as well as other hard surfaces.

The aqueous based bacterial inoculum remains in close, uniform contact in a “pseudo-biofilm” state with the treated material. The percent reduction in the surviving populations of challenge bacterial cells at 24 h versus those recovered from a non-treated control is determined.

The hydrophobic substrate may be repeatedly tested over time for assessment of persistent antimicrobial activity.

1. Scope

1.1 This test method is designed to evaluate (quantitatively) the antimicrobial effectiveness of agents incorporated or bound into or onto mainly flat (two dimensional) hydrophobic or polymeric surfaces. The method focuses primarily on assessing antibacterial activity; however, other microorganisms such as yeast and fungal conidia may be tested using this method.

1.2 The vehicle for the inoculum is an agar slurry which reduces the surface tension of the saline inoculum carrier and allows formation of a “pseudo-biofilm,” providing more even contact of the inoculum with the test surface.

Note 1—This test method facilitates the testing of hydrophobic surfaces by utilizing cells held in an agar slurry matrix. This test method, as written, is inappropriate to determine efficacy against biofilm cells, which are different both genetically and metabolically than planktonic cells used in this test.

1.3 This method can confirm the presence of antimicrobial activity in plastics or hydrophobic surfaces and allows determination of quantitative differences in antimicrobial activity between untreated plastics or polymers and those with bound or incorporated low water-soluble antimicrobial agents. Comparisons between the numbers of survivors on preservative-treated and control hydrophobic surfaces may also be made.

1.4 The procedure also permits determination of “shelf-life” or long term durability of an antimicrobial treatment which may be achieved through testing both non-washed and washed samples over a time span.

1.5 Knowledge of microbiological techniques is required for these procedures.

1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

ASTM Standards

E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents

E1428 Test Method for Evaluating the Performance of Antimicrobials in or on Polymeric Solids Against Staining by Streptoverticillium reticulum (A Pink Stain Organism)

G21 Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi

Keywords

agar slurry; hydrophobic surface; percent bacterial reduction; plastics; polymers; quantitative antibacterial assay; Agar detection; Antimicrobial formulations/testing; Effectiveness testing; Epoxy resins; Hydrophobic surfaces; Incorporated bound antimicrobials; Inoculum processing; Percent bacterial reduction; Polymers; Quantitative analysis/measurement--environmental; Quantitative antibacterial assay

ICS Code

ICS Number Code 07.100.01 (Microbiology in general)

Citing ASTM Standards

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