ASTM E2153 - 01(2011)

    Standard Practice for Obtaining Bispectral Photometric Data for Evaluation of Fluorescent Color

    Active Standard ASTM E2153 | Developed by Subcommittee: E12.05

    Book of Standards Volume: 06.01


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    ASTM E2153

    Significance and Use

    The bispectral or two-monochromator method is the definitive method for the determination of the general (illuminant-independent) radiation-transfer properties of fluorescent specimens (2). The Donaldson radiance factor is an instrument- and illuminant-independent photometric property of the specimen, and can be used to calculate its color for any desired illuminant and observer. The advantage of this method is that it provides a comprehensive characterization of the specimen's radiation-transfer properties, without the inaccuracies associated with source simulation and various methods of approximation.

    This practice provides a procedure for selecting the operating parameters of bispectrometers used for providing data of the desired precision. It also provides for instrument calibration by means of material standards, and for selection of suitable specimens for obtaining precision in the measurements.

    1. Scope

    1.1 This practice addresses the instrumental measurement requirements, calibration procedures, and material standards needed for obtaining precise bispectral photometric data for computing the colors of fluorescent specimens.

    1.2 This practice lists the parameters that must be specified when bispectral photometric measurements are required in specific methods, practices, or specifications.

    1.3 This practice applies specifically to bispectrometers, which produce photometrically quantitative bispectral data as output, useful for the characterization of appearance, as opposed to spectrofluorimeters, which produce instrument-dependent bispectral photometric data as output, useful for the purpose of chemical analysis.

    1.4 The scope of this practice is limited to the discussion of object-color measurement under reflection geometries; it does not include provisions for the analogous characterization of specimens under transmission geometries.

    This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    E179 Guide for Selection of Geometric Conditions for Measurement of Reflection and Transmission Properties of Materials

    E284 Terminology of Appearance

    E925 Practice for Monitoring the Calibration of Ultraviolet-Visible Spectrophotometers whose Spectral Bandwidth does not Exceed 2 nm

    E958 Practice for Measuring Practical Spectral Bandwidth of Ultraviolet-Visible Spectrophotometers

    E1164 Practice for Obtaining Spectrometric Data for Object-Color Evaluation

    E1341 Practice for Obtaining Spectroradiometric Data from Radiant Sources for Colorimetry

    E2152 Practice for Computing the Colors of Fluorescent Objects from Bispectral Photometric Data

    CIE Publications

    CIEReportofTC-2.25: Calibration Methods and Photoluminescent Standards for Total Radiance Factor Measurement

    NIST Publications

    NBSNo.260-66 Didymium Glass Filters for Calibrating the Wavelength Scale of Spectrophotometers Available from National Institute of Standards and Technology (NIST), 100 Bureau Dr., Stop 1070, Gaithersburg, MD 20899-1070, http://www.nist.gov.

    NPL Publications

    NPLReportMOM12 Problems of spectrofluorimetric standards for reflection and colorimetric use Available from National Physical Laboratory, Queens Road, Teddington, Middlesex, United Kingdom TW11 0LW, http://www.npl.co.uk/.


    ICS Code

    ICS Number Code 35.240.99 (IT applications in other fields)

    UNSPSC Code

    UNSPSC Code


    DOI: 10.1520/E2153-01R11

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