ASTM E2144 - 11

    Standard Practice for Personal Sampling and Analysis of Endotoxin in Metalworking Fluid Aerosols in Workplace Atmospheres

    Active Standard ASTM E2144 | Developed by Subcommittee: E34.50

    Book of Standards Volume: 11.03


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    Significance and Use

    Endotoxins in metalworking fluid aerosols present potential respiratory health hazards to workers who inhale them. Therefore, a consensus standard is needed to provide reliable data on workplace airborne endotoxin concentrations where metalworking fluids are used.

    This practice for measuring airborne endotoxin concentrations in metalworking fluid atmospheres will help to foster a better understanding of endotoxin exposure-response relationships.

    This practice facilitates comparisons of inter laboratory data from methods and field investigative studies.

    1. Scope

    1.1 This practice covers quantitative methods for the personal sampling and determination of bacterial endotoxin concentrations in poly-disperse metalworking fluid aerosols in workplace atmospheres. Users should have fundamental knowledge of microbiological techniques and endotoxin testing.

    1.2 Users of this practice may obtain personal or area exposure data of endotoxin in metalworking fluid aerosols, either on a short-term or full-shift basis in workplace atmospheres.

    1.3 This practice gives an estimate of the endotoxin concentration of the sampled atmosphere.

    1.4 This practice seeks to minimize inter laboratory variation but does not ensure uniformity of results.

    1.5 It is anticipated that this practice will facilitate inter laboratory comparisons of airborne endotoxin data from metalworking fluid atmospheres, particularly metal removal fluid atmospheres, by providing a basis for endotoxin sampling, extraction, and analytical methods.

    1.6 In 1997, the Occupational Safety and Health Administration (OSHA) empanelled a Standards Advisory Committee to make recommendations to the Administration regarding measures that the Administration could take to improve the health of workers exposed to metalworking fluids. A report to the Assistant Secretary of Labor for OSHA was submitted in July, 1999. Subcommittee E34.50 believes that the user community would benefit significantly if a standard method was developed to give the community guidance on a methodology for the sampling and analysis of personal airborne endotoxin exposure assessments in facilities using water-miscible metal removal fluids, based on the LAL assay or other endotoxin detection technologies as they become available.

    1.7 This practice does not attempt to set or imply limits for personal exposure to endotoxin in metalworking fluid aerosols in workplace environments.

    This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1356 Terminology Relating to Sampling and Analysis of Atmospheres

    D4840 Guide for Sample Chain-of-Custody Procedures

    D5337 Practice for Flow Rate Adjustment of Personal Sampling Pumps

    D6629 Guide for Selection of Methods for Estimating Soil Loss by Erosion

    E1370 Guide for Air Sampling Strategies for Worker and Workplace Protection

    E1497 Practice for Selection and Safe Use of Water-Miscible and Straight Oil Metal Removal Fluids

    E1542 Terminology Relating to Occupational Health and Safety

    OSHA Standards

    29CFR1910.1450 Occupational Exposure to Hazardous Chemicals in Laboratories

    Other Documents

    NIOSHManualofAnalyti


    ICS Code

    ICS Number Code 13.040.30 (Workplace atmospheres)

    UNSPSC Code

    UNSPSC Code 12161803(Aerosols)


    DOI: 10.1520/E2144-11

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