ASTM E1821 - 08

    Standard Test Method for Determination of Carbohydrates in Biomass by Gas Chromatography

    Active Standard ASTM E1821 | Developed by Subcommittee: E48.05

    Book of Standards Volume: 11.06


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    Significance and Use

    The structural carbohydrate content is used in conjunction with other assays to determine the total composition of biomass samples.

    1. Scope

    1.1 This test method describes the determination of structural carbohydrates present in a biomass sample, expressed as the percent mass of an oven-dried sample basis of each anhydrosugar.

    1.2 Sample materials suitable for this procedure include hard and softwoods, herbaceous materials, such as sericea and switchgrass, agricultural residues, such as corn stover, wheat straw, and bagasse, wastepaper, such as boxboard, office waste, and newsprint, acid or alkaline-pretreated biomass, washed free of any residual acid or alkali, and the solid fraction of fermentation residues.

    1.3 The options for the types of samples to be analyzed in this procedure are:

    1.3.1 Prepared Biomass Samples:

    1.3.1.1 Air Dried MaterialResults are reported as the percent by mass, based on the oven-dried mass of the air-dried sample.

    1.3.1.2 45°C Dried MaterialResults are reported as the percent by mass, based on the oven-dried mass of the 45°C dried sample.

    1.3.1.3 Freeze Dried MaterialResults are reported as the percent by mass, based on the oven-dried mass of the freeze dried sample.

    1.3.2 Extractives-Free SampleResults are reported as the percent by mass, based on the oven-dried mass of the extracted sample.

    1.4 This standard method is generally not suitable for samples that contain soluble, nonstructural carbohydrates unless they are removed prior to analysis.

    1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. See Section 8 for specific hazards statements.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1193 Specification for Reagent Water

    E1690 Test Method for Determination of Ethanol Extractives in Biomass

    E1721 Test Method for Determination of Acid-Insoluble Residue in Biomass

    E1756 Test Method for Determination of Total Solids in Biomass

    E1757 Practice for Preparation of Biomass for Compositional Analysis


    ICS Code

    ICS Number Code 65.040.20 (Buildings and installations for processing and storage of agricultural produce)

    UNSPSC Code

    UNSPSC Code 73101801(Biomass production services)


    DOI: 10.1520/E1821-08

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