ASTM E1758 - 01(2007)

    Standard Test Method for Determination of Carbohydrates in Biomass by High Performance Liquid Chromatography

    Active Standard ASTM E1758 | Developed by Subcommittee: E48.05

    Book of Standards Volume: 11.06


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    ASTM E1758

    Significance and Use

    The percentage, by mass, of sugar content is used in conjunction with other assays to determine the total composition of biomass samples.

    1. Scope

    1.1 This test method covers the determination of carbohydrates present in a biomass sample, expressed as the percent, by mass, of each sugar on a 105°C dried mass basis.

    Note 1—The percent sugar must be corrected for the water of hydrolysis before calculating the actual mass percent of the polysaccharide in the original biomass sample.

    1.2 Sample materials suitable for this procedure include hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), wastepaper (such as office waste, boxboard, and newsprint), acid or alkaline-pretreated biomass (washed free of any residual acid or alkali), and the solid fraction of fermentation residues. All results are reported relative to the 105°C oven-dried mass of the sample.

    1.3 The options for the types of samples to be analyzed in this test method are as follows:

    1.3.1 Prepared Biomass Samples:

    1.3.1.1 Air Dried (%Tad)The percent, by mass, of total solids of the air-dried sample.

    1.3.1.2 45°C Dried (%T45)The percent, by mass, of total solids of the 45°C dried sample.

    1.3.1.3 Freeze Dried (%Tfd)The percent, by mass, of total solids of the freeze dried sample.

    1.3.2 Extractives-Free Sample (%Text)The percent, by mass, of total solids of the extracted sample determined at 105°C.

    1.4 The values stated in SI units are to be regarded as the standard.

    1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Note 3 and Note 4.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1193 Specification for Reagent Water

    E1690 Test Method for Determination of Ethanol Extractives in Biomass

    E1721 Test Method for Determination of Acid-Insoluble Residue in Biomass

    E1756 Test Method for Determination of Total Solids in Biomass

    E1757 Practice for Preparation of Biomass for Compositional Analysis


    ICS Code

    ICS Number Code 65.040.20 (Buildings and installations for processing and storage of agricultural produce)

    UNSPSC Code

    UNSPSC Code 73101801(Biomass production services)


    DOI: 10.1520/E1758-01R07

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