ASTM E1566 - 00(2006)

    Standard Guide for Handling Hazardous Biological Materials in Liquid Nitrogen

    Active Standard ASTM E1566 | Developed by Subcommittee: E55.04

    Book of Standards Volume: 14.02


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    Significance and Use

    This guide is intended for use by individuals maintaining and handling hazardous biological material in liquid nitrogen freezers.

    This guide does not cover all aspects of every situation that may be encountered in maintaining hazardous biological material in liquid nitrogen; each situation must therefore be assessed individually using these guidelines.

    This guide is not intended for use with systems other than liquid nitrogen storage.

    This guide does not cover practices for preservation by freezing which are covered in Practice E 1342.

    1. Scope

    1.1 This guide covers recommended procedures for maintaining and handling hazardous biological materials at liquid nitrogen temperatures.

    1.2 This guide covers the safety precautions recommended when handling material stored in liquid nitrogen.

    1.3 This guide does not cover the maintenance and handling of hazardous biological materials maintained at cryogenic temperatures in systems other than liquid nitrogen.

    1.4 The values stated in SI units are to be regarded as the standard.

    This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    E1342 Practice for Preservation by Freezing, Freeze-Drying, and Low Temperature Maintenance of Bacteria, Fungi, Protista, Viruses, Genetic Elements, and Animal and Plant Tissues


    ICS Code

    ICS Number Code 07.080 (Biology. Botany. Zoology); 27.200 (Refrigerating technology)

    UNSPSC Code

    UNSPSC Code 60104000(Biotechnology and bio chemistry and genetics and microbiology and related materials); 24130000(Industrial refrigeration)


    Referencing This Standard

    DOI: 10.1520/E1566-00R06

    ASTM International is a member of CrossRef.

    Citation Format

    ASTM E1566-00(2006), Standard Guide for Handling Hazardous Biological Materials in Liquid Nitrogen, ASTM International, West Conshohocken, PA, 2006, www.astm.org

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