ASTM E1536 - 00(2006)

    Standard Practice for Detection of Mycoplasma Contamination of Bovine Serum by Large Volume Method

    Active Standard ASTM E1536 | Developed by Subcommittee: E55.04

    Book of Standards Volume: 14.02


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    Significance and Use

    Mycoplasmas of bovine origin are prevalent contaminants of cell cultures. Contamination can be detected by the large volume method.3 ,4

    Heat inactivated serum need not be tested for mycoplasmas. Heating serum to 56°C for 30 min will kill mycoplasmas.

    Mycoplasmas may be present in any particular lot of serum but may not be detected because of inadequate sample size; thus, negative test results do not provide absolute assurance that the test serum is free of mycoplasmas.

    1. Scope

    1.1 This practice covers the procedures used for detection of mycoplasma contamination in serum by direct microbiological culture.

    1.2 This practice does not cover procedures used for detection of mycoplasma in cell cultures.

    1.3 This practice does not cover indirect methods for detection of mycoplasma contamination.

    1.4 This practice does not cover methods for identification of mycoplasma cultures.

    This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    E1531 Practice for Detection of Mycoplasma Contamination of Cell Cultures by Growth on Agarose Medium


    ICS Code

    ICS Number Code 07.100.10 (Medical microbiology)

    UNSPSC Code

    UNSPSC Code 60104000(Biotechnology and bio chemistry and genetics and microbiology and related materials)


    DOI: 10.1520/E1536-00R06

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