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This practice covers the procedures used for detection of mycoplasma contamination in serum by direct microbiological culture.
Formerly under the jurisdiction of Committee E55 on Manufacture of Pharmaceutical Products, this practice was withdrawn in August 2014. This standard was withdrawn without replacement due to its limited use by the industry.
1.1 This practice covers the procedures used for detection of mycoplasma contamination in serum by direct microbiological culture.
1.2 This practice does not cover procedures used for detection of mycoplasma in cell cultures.
1.3 This practice does not cover indirect methods for detection of mycoplasma contamination.
1.4 This practice does not cover methods for identification of mycoplasma cultures.
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
E1531 Practice for Detection of Mycoplasma Contamination of Cell Cultures by Growth on Agarose Medium
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ASTM E1536-00(2006), Standard Practice for Detection of Mycoplasma Contamination of Bovine Serum by Large Volume Method (Withdrawn 2014), ASTM International, West Conshohocken, PA, 2006, www.astm.orgBack to Top