ASTM E1532 - 00(2006)

    Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of Bisbenzamide DNA-Binding Fluorochrome

    Active Standard ASTM E1532 | Developed by Subcommittee: E55.04

    Book of Standards Volume: 14.02


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    ASTM E1532

    Significance and Use

    Mycoplasma hyorhinis, cultivar α strains (1)3 do not grow on any of the standard media used for mycoplasma cultivation. These strains, which are found as contaminants in cell cultures, are detected by indirect methods.

    A specialized medium has been described but it is not yet in wide use (2).

    This practice should be used in conjunction with Practice E 1531.

    All cell cultures to be examined for mycoplasma should undergo a minimum of two passages in antibiototic-free tissue culture medium before testing.

    1. Scope

    1.1 This practice covers the use of cell cultures and DNA-binding flurorochrome techniques to detect mycoplasma contamination of cell cultures.

    1.2 This practice does not cover axenic cultivation or identification of mycoplasmas.

    This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    E1531 Practice for Detection of Mycoplasma Contamination of Cell Cultures by Growth on Agarose Medium


    ICS Code

    ICS Number Code 07.100.10 (Medical microbiology)

    UNSPSC Code

    UNSPSC Code 60104000(Biotechnology and bio chemistry and genetics and microbiology and related materials)


    DOI: 10.1520/E1532-00R06

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