ASTM E1531 - 00(2006)

    Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Growth on Agarose Medium

    Active Standard ASTM E1531 | Developed by Subcommittee: E55.04

    Book of Standards Volume: 14.02


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    ASTM E1531

    Abstract

    This practice covers the procedures used for detection of mycoplasma contamination of cell cultures by growth on agarose medium. This practice does not cover identification of mycoplasma and indirect methods for detection of mycoplasma. This practice will not detect cultivar strains of Mycoplasma hyorhinis nor intended for use in detection of mycoplasma contamination in sera, culture media, vaccines, or other systems. The practice involves DM-1 solid medium preparation, quality control, and mycoplasma isolation.

    This abstract is a brief summary of the referenced standard. It is informational only and not an official part of the standard; the full text of the standard itself must be referred to for its use and application. ASTM does not give any warranty express or implied or make any representation that the contents of this abstract are accurate, complete or up to date.

    1. Scope

    1.1 This practice covers the procedures used for detection of mycoplasma contamination by direct microbiological culture.

    1.2 This practice does not cover indirect methods for detection of mycoplasma such as DNA staining, biochemical detection, or genetic probes.

    1.3 This practice does not cover methods for identification of mycoplasma organisms.

    1.4 This practice will not detect cultivar strains (1) of Mycoplasma hyorhinis.

    1.5 This practice is not intended for use in detection of mycoplasma contamination in sera, culture media, vaccines, or other systems.

    This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    E1532 Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of Bisbenzamide DNA-Binding Fluorochrome


    ICS Code

    ICS Number Code 07.100.01 (Microbiology in general)

    UNSPSC Code

    UNSPSC Code 60104000(Biotechnology and bio chemistry and genetics and microbiology and related materials)


    DOI: 10.1520/E1531-00R06

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