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Significance and Use
4.1 This practice is to be used for the removal of virucidal agents from test product-virus mixtures, or from test product-neutralizer-virus mixtures, at or after the contact period and before the inoculation of these mixtures into host systems for assay of viral infectivity.
4.2 The purpose of the practice is to reduce the concentration of the cytotoxic properties of the test product and neutralizers in order to permit the evaluation of viral infectivity at dilutions that would otherwise be toxic to the host cells.
1.1 This practice is intended to be used to reduce the cytotoxic level of the virus-test product mixture prior to assaying for viral infectivity. It is used in conjunction with evaluations of the virucidal efficacy of disinfectant solutions, wipes, trigger sprays, or pressurized disinfectant spray products intended for use on inanimate, nonporous environmental surfaces. This practice may also be used in the evaluation of hygienic handwashes/handrubs, or for other special applications. The practice may be employed with all viruses and host systems.
1.3 This practice utilizes gel filtration technology. The effectiveness of the practice is dependent on the ratio of gel bed volume to sample size and uniformity in the preparation of columns as well as the conditions of entrifugation. The effectiveness of this practice is maximized by investigator practice and experience with gel filtration techniques.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
E1052 Test Method to Assess the Activity of Microbicides against Viruses in Suspension
E1053 Test Method to Assess Virucidal Activity of Chemicals Intended for Disinfection of Inanimate, Nonporous Environmental Surfaces
ICS Number Code 11.080.20 (Disinfectants and antiseptics); 71.100.35 (Chemicals for industrial and domestic desinfection purposes)
UNSPSC Code 60104000(Biotechnology and bio chemistry and genetics and microbiology and related materials)
|Link to Active (This link will always route to the current Active version of the standard.)|
ASTM E1482-12, Standard Practice for Use of Gel Filtration Columns for Cytotoxicity Reduction and Neutralization, ASTM International, West Conshohocken, PA, 2012, www.astm.orgBack to Top