ASTM E1303 - 95(2010)

    Practice for Refractive Index Detectors Used in Liquid Chromatography

    Active Standard ASTM E1303 | Developed by Subcommittee: E13.19

    Book of Standards Volume: 03.06


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    Significance and Use

    Although it is possible to observe and measure each of several characteristics of a detector under different and unique conditions, it is the intent of this practice that a complete set of detector test results should be obtained under the same operating conditions. It should also be noted that to specify completely a detector's capability, its performance should be measured at several sets of conditions within the useful range of the detector.

    The objective of this practice is to test the detector under specified conditions and in a configuration without an LC column. This is a separation independent test. In certain circumstances it might also be necessary to test the detector in the separation mode with an LC column in the system, and the appropriate concerns are also mentioned. The terms and tests described in this practice are sufficiently general so that they may be adapted for use at whatever conditions may be chosen for other reasons.

    1. Scope

    1.1 This practice covers tests used to evaluate the performance and to list certain descriptive specifications of a refractive index (RI) detector used as the detection component of a liquid chromatographic (LC) system.

    1.2 This practice is intended to describe the performance of the detector both independent of the chromatographic system (static conditions, without flowing solvent) and with flowing solvent (dynamic conditions).

    1.3 The values stated in SI units are to be regarded as the standard.

    1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    E386 Practice for Data Presentation Relating to High-Resolution Nuclear Magnetic Resonance (NMR) Spectroscopy


    ICS Code

    ICS Number Code 17.180.30 (Optical measuring instruments); 71.040.50 (Physicochemical methods of analysis)

    UNSPSC Code

    UNSPSC Code


    DOI: 10.1520/E1303-95R10

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