ASTM E1279-89(2008)

    Standard Test Method for Biodegradation By a Shake-Flask Die-Away Method (Withdrawn 2013)

    Withdrawn Standard: ASTM E1279-89(2008) | Developed by Subcommittee: E47.04

    WITHDRAWN, NO REPLACEMENT


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    Withdrawn Rationale:

    This test method describes procedures for assessing the biodegradation of chemicals in natural surface water samples.

    Formerly under the jurisdiction of Committee E47 on Biological Effects and Environmental Fate, this test method was withdrawn in March 2013. This standard is being withdrawn without replacement due to its limited use by industry.

    1. Scope

    1.1 This test method describes procedures for assessing the biodegradation of chemicals in natural surface water samples.

    1.2 This test method provides an opportunity to evaluate rates of biodegradation in the presence and absence of natural sediment materials. It also may provide limited information on the abiotic degradation rate, and sorption to sediment and vessel walls.

    1.3 This test method allows for the development of a first-order rate constant, based on the disappearance of the test compound with time, and a second-order rate constant, normalized for changes in microbial biomass.

    1.4 This test method requires a chemical specific analytical method and the concentrations of test substance employed are dependent on the sensitivity of the analytical method.

    1.5 This test method is designed to be applicable to compounds that are not inhibitory to bacteria at the concentrations used in the test method, which do not rapidly volatilize from water, that are soluble at the initial test concentration and that do not degrade rapidly by abiotic processes, such as hydrolysis.

    1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.



    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D1193 Specification for Reagent Water

    D4129 Test Method for Total and Organic Carbon in Water by High Temperature Oxidation and by Coulometric Detection

    E895 Practice for Determination of Hydrolysis Rate Constants of Organic Chemicals in Aqueous Solutions

    E896 Test Method for Conducting Aqueous Direct Photolysis Tests

    E1194 Test Method for Vapor Pressure

    E1195 Test Method for Determining a Sorption Constant (Koc) for an Organic Chemical in Soil and Sediments


    DOI: 10.1520/E1279-89R08

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