ASTM E1241 - 05(2013)

    Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes

    Active Standard ASTM E1241 | Developed by Subcommittee: E50.47

    Book of Standards Volume: 11.06


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    Significance and Use

    5.1 Protection of a species requires prevention of unacceptable effects on the number, weight, health, and uses of the individuals of that species. An early life-stage toxicity test provides information about the chronic toxicity of a test material to a species of fish. The primary adverse effects studied are reduced survival and growth.

    5.2 Results of early life-stage toxicity tests are generally useful estimates of the results of comparable life-cycle tests with the same species (1).4 However, results of early life-stage tests are sometimes under estimative of those obtained with the same species in the longer life-cycle tests (2).

    5.3 Results of early life-stage toxicity tests might be used to predict long-term effects likely to occur on fish in field situations as a result of an exposure under comparable conditions, except that motile organisms might avoid exposure when possible.

    5.4 Results of early life-stage toxicity tests might be used to compare the chronic sensitivities of different fish species and the chronic toxicities of different materials, and to study the effects of various environmental factors on results of such tests.

    5.5 Results of early life-stage toxicity tests might be an important consideration when assessing the hazards of materials to aquatic organisms (see Guide E1023) or when deriving water quality criteria for aquatic organisms (3).

    5.6 Results of an early life-stage test might be useful for predicting the results of chronic tests on the same test material with the same species in another water or with another species in the same or a different water. Most such predictions take into account the results of acute toxicity tests, and so the usefulness of the results of an early life-stage test is greatly increased by reporting also the results of an acute toxicity test (see Guide E729) conducted with juveniles of the same species under the same conditions.

    5.7 Results of early life-stage toxicity tests might be useful for studying the biological availability of, and structure-activity relationships between, test materials.

    5.8 Results of early life-stage toxicity tests will depend on temperature, composition of the dilution water, condition of the test organisms, and other factors.

    1. Scope

    1.1 This guide describes procedures for obtaining laboratory data concerning the adverse effects of a test material added to dilution water—but not to food—on certain species of freshwater and saltwater fishes during 28 to 120-day (depending on species) continuous exposure, beginning before hatch and ending after hatch, using the flow-through technique. This guide will probably be useful for conducting early life-stage toxicity tests with some other species of fish, although modifications might be necessary.

    1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting early life-stage toxicity tests with fishes.

    1.3 These procedures are applicable to all chemicals, either individually or in formulations, commercial products, or known mixtures, that can be measured accurately at the necessary concentrations in water. With appropriate modifications these procedures can be used to conduct tests on temperature, dissolved oxygen, and pH and on such materials as aqueous effluents (see Guide E1192), leachates, oils, particulate matter, sediments, and surface waters.

    1.4 This guide is arranged as follows:


     

    Section

     

    Referenced Documents

    2

    Terminology

    3

    Summary of Standard

    4

    Significance and Use

    5.1

    Hazards

    6

    Apparatus

    7

     Facilities

    7.1

     Construction Materials

    7.2

     Metering System

    7.3

     Test Chambers and Incubation Cups

    7.4

     Cleaning

    7.5

     Acceptability

    7.6

    Dilution Water

    8

     Requirements

    8.1

     Source

    8.2

     Treatment

    8.3

     Characterization

    8.4

    Test Material

    9

     General

    9.1

     Stock Solution

    9.2

     Test Concentration(s)

    9.3

    Test Organisms

    10

     Species

    10.1

     Age

    10.2

     Source

    10.3

     Brood Stock

    10.4

     Handling

    10.5

    Procedure

    11

     Experimental Design

    11.1

     Dissolved Oxygen

    11.2

     Temperature

    11.3

     Beginning the Test

    11.4

     Thinning

    11.5

     Feeding

    11.6

     Duration of Test

    11.7

     Biological Data

    11.8

     Other Measurements

    11.9

    Analytical Methodology

    12

    Acceptability of Test

    13

    Calculation of Results

    14

    Documentation

    15

    Appendixes

     

    Appendix X1 Salmon, Trout, and Char

     

    Appendix X2 Northern pike

     

    Appendix X3 Fathead minnow

     

    Appendix X4 White sucker

     

    Appendix X5 Channel catfish

     

    Appendix X6 Bluegill

     

    Appendix X7 Gulf toadfish

     

    Appendix X8 Sheepshead minnow

     

    Appendix X9 Silversides

     

    Appendix X10 Statistical Guidance

     

    Appendix X11. Striped Bass




    1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 6 and 9.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    E729 Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians

    E943 Terminology Relating to Biological Effects and Environmental Fate

    E1023 Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses

    E1192 Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians

    E1203 Practice for Using Brine Shrimp Nauplii as Food for Test Animals in Aquatic Toxicology


    ICS Code

    ICS Number Code 65.150 (Fishing and fish breeding)

    UNSPSC Code

    UNSPSC Code 50121500(Fish); 77101500(Environmental impact assessment)


    DOI: 10.1520/E1241-05R13

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