Withdrawn Rationale:
This practice describes procedures for hatching, harvesting, and testing the acceptability of brine shrimp nauplii for use as a food for certain fish and invertebrate species that are used in aquatic toxicity tests.
Formerly under the jurisdiction of Committee E47 on Biological Effects and Environmental Fate, this practice was withdrawn in March 2013. This standard is being withdrawn without replacement due to its limited use by industry.
1. Scope
1.1 This practice describes procedures for hatching, harvesting, and testing the acceptability of brine shrimp nauplii for use as a food for certain fish and invertebrate species that are used in aquatic toxicity tests. The term “brine shrimp” refers to all species in the genus Artemia (1) although this practice specifically deals only with those species for which cysts (encysted embryos) are commercially available.
1.2 These procedures are applicable to all brine shrimp nauplii that are obtained by incubating commercially available cysts. With appropriate processing, cysts collected by noncommercial harvesters can be subjected to these same procedures.
1.3 Modification of these procedures might be justified by special needs or circumstances.
1.4 This practice is organized as follows:
| | Section |
| Referenced Documents | 2 |
| Terminology | 3 |
| Summary of Practice | 4 |
| Significance and Use | 5 |
| Hazards | 6 |
| Obtaining Cysts | 7 |
| Selecting a Source | 7.1 |
| Storage | 7.2 |
| Hatching Cysts | 8 |
| Harvesting Nauplii | 9 |
| Testing Nauplii | 10 |
| Feeding Assay | 10.1 |
| Chemical and Physical Measurements | 10.2 |
| Using Nauplii | 11 |
| Report | 12 |
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 6.
2. Referenced Documents (purchase separately) 
The documents listed below are referenced within the subject standard but are not provided as part of the standard.
ASTM Standards
E1191 Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids
E1241 Guide for Conducting Early Life-Stage Toxicity Tests with Fishes
Keywords
aquatic toxicology; Artemia; brine shrimp; feeding; nutrition;
DOI: 10.1520/E1203-98R12
ASTM International is a member of CrossRef.
Citing ASTM Standards
[Back to Top]
