ASTM E1191 - 03a(2008)

    Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids

    Active Standard ASTM E1191 | Developed by Subcommittee: E50.47

    Book of Standards Volume: 11.06


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    Significance and Use

    Protection of a species requires prevention of unacceptable effects on the number, weight, health, and uses of the individuals of that species. A life-cycle toxicity test is conducted to determine what changes in the numbers and weights of individuals of the test species result from effects of the test material on survival, growth, and reproduction. Information might also be obtained on effects of the material on the health and uses of the species.

    Results of life-cycle tests with mysids might be used to predict long-term effects likely to occur on mysids in field situations as a result of exposure under comparable conditions.

    Results of life-cycle tests with mysids might be used to compare the chronic sensitivities of different species and the chronic toxicities of different materials, and also to study the effects of various environmental factors on results of such tests.

    Results of life-cycle tests with mysids might be an important consideration when assessing the hazards of materials to aquatic organisms (see Guide E 1023) or when deriving water quality criteria for aquatic organisms (1).3

    Results of a life-cycle test with mysids might be useful for predicting the results of chronic tests on the same test material with the same species in another water or with another species in the same or a different water (2). Most such predictions take into account results of acute toxicity tests, and so the usefulness of the results from a life-cycle test with mysids is greatly increased by also reporting the results of an acute toxicity test (see Guide E 729) conducted under the same conditions.

    Results of life-cycle tests with mysids might be useful for studying the biological availability of, and structure-activity relationships between, test materials.

    Results of life-cycle tests with mysids might be useful for predicting population effects on the same species in another water or with another species in the same or a different water (3).

    1. Scope

    1.1 This guide describes procedures for obtaining laboratory data concerning the adverse effects of a test material added to dilution water, but not to food, on certain species of saltwater mysids during continuous exposure from immediately after birth until after the beginning of reproduction using the flow-through technique. These procedures will probably be useful for conducting life-cycle toxicity tests with other species of mysids, although modifications might be necessary.

    1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information on new concepts and procedures for conducting life-cycle toxicity tests with saltwater mysids.

    1.3 These procedures are applicable to all chemicals, either individually or in formulations, commercial products, or known mixtures, that can be measured accurately at the necessary concentrations in water. With appropriate modifications, these procedures can be used to conduct tests on temperature, dissolved oxygen, and pH and on such materials as aqueous effluents (see also Guide E 1192), leachates, oils, particulate matter, sediments, and surface waters.

    1.4 This guide is arranged as follows:

    Section
    Referenced Documents2
    Terminology3
    Summary of Guide4
    Significance and Use5
    Hazards7
    Apparatus6
    Facilities6.1
    Construction Materials6.2
    Metering System6.3
    Test Chambers6.4
    Cleaning6.5
    Acceptability6.6
    Dilution Water8
    Requirements8.1
    Source8.2
    Treatment8.3
    Characterization8.4
    Test Material9
    General9.1
    Stock Solution9.2
    Test Concentration(s)9.3
    Test Organisms10
    Species10.1
    Age10.2
    Source10.3
    Brood Stock10.4
    Food10.5
    Handling10.6
    Harvesting Young10.7
    Quality10.8
    Procedure11
    Experimental Design11.1
    Dissolved Oxygen11.2
    Temperature11.3
    Beginning the Test11.4
    Feeding11.5
    Cleaning11.6
    Duration of Test11.7
    Biological Data11.8
    Other Measurements11.9
    Analytical Methodology12
    Acceptability of Test13
    Calculation14
    Documentation15
    Keywords16
    Appendix
    X1. Statistical Guidance

    1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 7.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    E729 Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians

    E943 Terminology Relating to Biological Effects and Environmental Fate

    E1023 Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses

    E1192 Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians

    E1203 Practice for Using Brine Shrimp Nauplii as Food for Test Animals in Aquatic Toxicology


    ICS Code

    ICS Number Code 13.300 (Protection against dangerous goods)

    UNSPSC Code

    UNSPSC Code 77101500(Environmental impact assessment)


    DOI: 10.1520/E1191-03AR08

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